RPL39 Polyclonal antibody

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• RPL39 Polyclonal antibody (14990-1-AP)

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• KD/KO Validated

RPL39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14990-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ribosomal protein L39, Large ribosomal subunit protein eL39, eL39, 60S ribosomal protein L39

• 

  WB analysis of HepG2 using 14990-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14990-1-AP (RPL39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 14990-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14990-1-AP (RPL39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 14990-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14990-1-AP (RPL39 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 14990-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14990-1-AP (RPL39 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14990-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL39 antibody (14990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, human liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14990-1-AP targets RPL39 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6975

  Product name: Recombinant human RPL39 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-51 aa of BC001019

  Sequence: MSSHKTFRIKRFLAKKQKQNRPIPQWIRMKTGNKIRYNSKRRHWRRTKLGL

• Full Name: ribosomal protein L39
• Calculated Molecular Weight: 6 kDa
• Observed Molecular Weight: 6 kDa
• GenBank Accession Number: BC001019
• Gene Symbol: RPL39
• Gene ID (NCBI): 6170
• RRID: AB_2238670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62891
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPL39 Polyclonal antibody (Cat# 14990-1-AP) has 9 citations published in journals including Cell Discov, Mol Cell, Front Immunol, Transl Res, Front Endocrinol, iScience, Int J Biochem Cell Biol, J Cancer Res Clin Oncol, and Elife. Research fields span ribosome biology, chromosome clustering, nascent polypeptide scanning, glioma prognosis and molecular subtyping, microvesicle communication in myeloma, spermatogenesis, proteostasis, cell proliferation, and zebrafish heart regeneration.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
human	WB	Mol Cell; Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC.; Authors - Martin Gamerdinger
human	WB,IHC,IF	Front Immunol; Identification and validation of a 17-gene signature to improve the survival prediction of gliomas; Authors - Shiao Tong ; KD Validated
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	IHC	Front Endocrinol (Lausanne); An integrated transcriptomic analysis unveils the regulatory roles of RNA binding proteins during human spermatogenesis; Authors - Ning Xu
mouse	WB,IP,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou ; KO Validated