|Positive WB detected in||MCF7 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15340-1-AP targets RPL7A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPL7A fusion protein Ag7543|
|Full Name||ribosomal protein L7a|
|Calculated molecular weight||266 aa, 30 kDa|
|Observed molecular weight||30-32 kDa|
|GenBank accession number||BC005128|
|Gene ID (NCBI)||6130|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].
|Product Specific Protocols|
|WB protocol for RPL7A antibody 15340-1-AP||Download protocol|
|IHC protocol for RPL7A antibody 15340-1-AP||Download protocol|
|IF protocol for RPL7A antibody 15340-1-AP||Download protocol|
|IP protocol for RPL7A antibody 15340-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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