RPL7A Polyclonal antibody

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• RPL7A Polyclonal antibody (15340-1-AP)

RPL7A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15340-1-AP Copied!

Publications (17)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

60S ribosomal protein L7a, Large ribosomal subunit protein eL8, PLA X polypeptide, PLA-X polypeptide, ribosomal protein L7a

• 

  WB analysis of MCF-7 using 15340-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 15340-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 15340-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat kidney using 15340-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 15340-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse kidney using 15340-1-AP

  IP result of anti-RPL7A (IP:15340-1-AP, 4ug; Detection:15340-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15340-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL7A antibody 15340-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF7 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15340-1-AP targets RPL7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7543

  Product name: Recombinant human RPL7A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-266 aa of BC005128

  Sequence: MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG

• Full Name: ribosomal protein L7a
• Calculated Molecular Weight: 266 aa, 30 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC005128
• Gene Symbol: RPL7A
• Gene ID (NCBI): 6130
• RRID: AB_2254051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62424
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].

Publications

What published studies show

RPL7A Polyclonal Antibody (Cat# 15340-1-AP) has accumulated 17 citations across high-impact journals including Cell, Nature Communications, Molecular Cell, Nucleic Acids Research, Journal of Experimental Medicine, Oncogene, JCI Insight, and Pain. Associated research fields encompass ribosome biogenesis and translational control, post-transcriptional RNA regulation, inflammation and autoimmunity, cancer progression, neurodegeneration, ferroptosis, and pain signaling.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Cell; Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.; Authors - Takashi Mino
human	IF	Nat Commun; Nuclear and cytoplasmic specific RNA binding proteome enrichment and its changes upon ferroptosis induction; Authors - Haofan Sun
mouse	IF	Nat Commun; RiboBright reveals cell-type-specific differences in ribosome organization and movement.; Authors - Georgia Pouladofonou
mouse	WB	Nat Commun; DDX41 resolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death; Authors - Honghao Bi
human	IP	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	Nucleic Acids Res; The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis; Authors - Matthew John Eastham