RPL7A Polyclonal antibody

RPL7A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15340-1-AP

Synonyms

60S ribosomal protein L7a, PLA X polypeptide, ribosomal protein L7a, RPL7A, SURF 3, SURF3, Surfeit locus protein 3, TRUP



Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15340-1-AP targets RPL7A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL7A fusion protein Ag7543
Full Name ribosomal protein L7a
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC005128
Gene symbol RPL7A
Gene ID (NCBI) 6130
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].

Protocols

Product Specific Protocols
WB protocol for RPL7A antibody 15340-1-APDownload protocol
IHC protocol for RPL7A antibody 15340-1-APDownload protocol
IF protocol for RPL7A antibody 15340-1-APDownload protocol
IP protocol for RPL7A antibody 15340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Exp Med

Phosphorylation-dependent Regnase-1 release from endoplasmic reticulum is critical in IL-17 response.

Authors - Hiroki Tanaka
humanIP

Mol Cell

Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.

Authors - Dianrong Li
mouseWB, IF

Cell

Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.

Authors - Takashi Mino