RPL7A Polyclonal antibody

RPL7A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 15340-1-AP

Synonyms

SURF 3, ribosomal protein L7a, PLA-X polypeptide, PLA X polypeptide, Large ribosomal subunit protein eL8

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Validation Data Gallery

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  WB analysis of MCF-7 using 15340-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15340-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15340-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15340-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15340-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15340-1-AP

  IP result of anti-RPL7A (IP:15340-1-AP, 4ug; Detection:15340-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human breast cancer using 15340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15340-1-AP

  Immunofluorescent analysis of HepG2 cells, using RPL7A antibody 15340-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: MCF7 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

15340-1-AP targets RPL7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL7A fusion protein Ag7543
• Full Name: ribosomal protein L7a
• Calculated Molecular Weight: 266 aa, 30 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC005128
• Gene Symbol: RPL7A
• Gene ID (NCBI): 6130
• RRID: AB_2254051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254].

Publications

Species	Application	Title
mouse	WB, IF	Cell; Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.; Authors - Takashi Mino
human	IP	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	Nucleic Acids Res; The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis; Authors - Matthew John Eastham
human	WB	Am J Cancer Res; UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc; Authors - Dalian Gong
human	IF	Nat Commun; Nuclear and cytoplasmic specific RNA binding proteome enrichment and its changes upon ferroptosis induction; Authors - Haofan Sun