RPN1 Polyclonal antibody

RPN1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12894-1-AP

Synonyms

hrd2, OST1, RBPH1, Ribophorin 1, ribophorin I, RPN I, RPN1



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inmouse lung tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

12894-1-AP targets RPN1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPN1 fusion protein Ag3576
Full Name ribophorin I
Calculated molecular weight 69 kDa
Observed molecular weight 66-69 kDa
GenBank accession numberBC010839
Gene symbol RPN1
Gene ID (NCBI) 6184
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPN1 antibody 12894-1-APDownload protocol
IHC protocol for RPN1 antibody 12894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Authors - Yini Wang
humanWB

Nat Chem Biol

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Authors - Cecilia Lopez-Sambrooks
yeastWB

Nature

Reconfiguration of the proteasome during chaperone-mediated assembly.

Authors - Soyeon Park