RPN1 Polyclonal antibody

RPN1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12894-1-AP

Synonyms

hrd2, OST1, RBPH1, Ribophorin 1, ribophorin I, RPN I, RPN1



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inmouse lung tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

12894-1-AP targets RPN1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPN1 fusion protein Ag3576
Full Name ribophorin I
Calculated molecular weight 69 kDa
Observed molecular weight 66-69 kDa
GenBank accession numberBC010839
Gene symbol RPN1
Gene ID (NCBI) 6184
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPN1 antibody 12894-1-APDownload protocol
IHC protocol for RPN1 antibody 12894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.

Authors - Wenjuan Zhang
humanWB

Proteomics

Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Authors - Yini Wang
humanWB

Nat Chem Biol

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Authors - Cecilia Lopez-Sambrooks
humanWB

Sci Adv

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Authors - Chatchai Phoomak
yeastWB

Nature

Reconfiguration of the proteasome during chaperone-mediated assembly.

Authors - Soyeon Park