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  • KD/KO Validated

RPN2 Polyclonal antibody

RPN2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10576-1-AP

Synonyms

RIBIIR, Ribophorin 2, ribophorin II, RPN II, RPN2, RPNII, SWP1



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10576-1-AP targets RPN2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPN2 fusion protein Ag0862
Full Name ribophorin II
Calculated molecular weight 69 kDa
Observed molecular weight 68-69 kDa
GenBank accession numberBC003560
Gene symbol RPN2
Gene ID (NCBI) 6185
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPN2 (Ribophorin II), an integral rough ER membrane glycoprotein, is part of an N-oligosaccharyl transferase complex and is involved in translocation and the maintenance of the structural uniqueness of the rough ER. RPN2 expression has been reported to be significantly associated with solid cancers, including breast cancer, esophageal squamous cell carcinoma, colorectal cancer, and lung squamous cell carcinoma.

Protocols

Product Specific Protocols
WB protocol for RPN2 antibody 10576-1-APDownload protocol
IHC protocol for RPN2 antibody 10576-1-APDownload protocol
IP protocol for RPN2 antibody 10576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Onco Targets Ther

Downregulation of ribophorin II suppresses tumor growth, migration, and invasion of nasopharyngeal carcinoma.

Authors - Feilong Hong
  • KD Validated
humanIHC

Onco Targets Ther

RPN2 Predicts Poor Prognosis and Promotes Bladder Cancer Growth and Metastasis via the PI3K-Akt Pathway.

Authors - Chenglin Han