RPP21 Polyclonal antibody

• 

  WB analysis of COLO 320 using 16377-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16377-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of COLO 320 using 16377-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16377-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RPP21 Polyclonal Antibody for WB, ELISA

Cat No. 16377-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C6orf135, CAT60, Ribonucleoprotein V, RNaseP protein p21, RPP21

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Tested Applications

• Positive WB detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16377-1-AP targets RPP21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9353

  Product name: Recombinant human RPP21 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-154 aa of BC011730

  Sequence: MAGPVKDREAFQRLNFLYQAAHCVLAQDPENQALARFYCYTERTIAKRLVLRRDPSVKRTLCRGCSSLLVPGLTCTHRQRRCRGQRWTVQTCLTCQRSQRFLNDPGHLLWGDRPEAQLGSQADSKPLQPLPNTAHSISDRLPEEKMQTQGSSNQ

• Full Name: ribonuclease P/MRP 21kDa subunit
• Calculated Molecular Weight: 154 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC011730
• Gene Symbol: RPP21
• Gene ID (NCBI): 79897
• RRID: AB_2878250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H633
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	bioRxiv; Composition and RNA binding specificity of metazoan RNase MRP; Authors - Yuan Liu