RPP21 Polyclonal antibody

RPP21 Polyclonal Antibody for WB, ELISA

Cat No. 16377-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C6orf135, CAT60, Ribonucleoprotein V, RNaseP protein p21, RPP21

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis of COLO 320 using 16377-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of COLO 320 using 16377-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of COLO 320 using 16377-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of COLO 320 using 16377-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

COLO 320 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

16377-1-AP targets RPP21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9353 Product name: Recombinant human RPP21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC011730 Sequence: MAGPVKDREAFQRLNFLYQAAHCVLAQDPENQALARFYCYTERTIAKRLVLRRDPSVKRTLCRGCSSLLVPGLTCTHRQRRCRGQRWTVQTCLTCQRSQRFLNDPGHLLWGDRPEAQLGSQADSKPLQPLPNTAHSISDRLPEEKMQTQGSSNQ Predict reactive species
Full Name	ribonuclease P/MRP 21kDa subunit
Calculated Molecular Weight	154 aa, 18 kDa
Observed Molecular Weight	18 kDa
GenBank Accession Number	BC011730
Gene Symbol	RPP21
Gene ID (NCBI)	79897
RRID	AB_2878250
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H633
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for RPP21 antibody 16377-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
	WB	Nat Commun Reversible proliferative arrest induced by rapid depletion of RNase MRP Authors - Yuan Liu View Article
		Nucleic Acids Res Composition and RNA binding specificity of metazoan RNase MRP Authors - Yuan Liu View Article
human	WB	bioRxiv Composition and RNA binding specificity of metazoan RNase MRP Authors - Yuan Liu View Article