RPP21 Polyclonal antibody

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• RPP21 Polyclonal antibody (16377-1-AP)

RPP21 Polyclonal Antibody for WB, ELISA

Cat No. 16377-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C6orf135, CAT60, Ribonucleoprotein V, RNaseP protein p21, RPP21

• 

  WB analysis of COLO 320 using 16377-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 16377-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 16377-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 16377-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16377-1-AP targets RPP21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9353

  Product name: Recombinant human RPP21 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-154 aa of BC011730

  Sequence: MAGPVKDREAFQRLNFLYQAAHCVLAQDPENQALARFYCYTERTIAKRLVLRRDPSVKRTLCRGCSSLLVPGLTCTHRQRRCRGQRWTVQTCLTCQRSQRFLNDPGHLLWGDRPEAQLGSQADSKPLQPLPNTAHSISDRLPEEKMQTQGSSNQ

• Full Name: ribonuclease P/MRP 21kDa subunit
• Calculated Molecular Weight: 154 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC011730
• Gene Symbol: RPP21
• Gene ID (NCBI): 79897
• RRID: AB_2878250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H633
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPP21 Polyclonal antibody (Cat# 16377-1-AP) has 3 citations. These appear in Nature Communications, Nucleic Acids Research, and bioRxiv. The associated research fields include RNase MRP biology, RNA binding specificity in metazoans, and reversible proliferative arrest induced by RNase MRP depletion. The antibody was primarily used in Western Blot (WB) applications, with one study also examining human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Nat Commun; Reversible proliferative arrest induced by rapid depletion of RNase MRP; Authors - Yuan Liu
		Nucleic Acids Res; Composition and RNA binding specificity of metazoan RNase MRP; Authors - Yuan Liu
human	WB	bioRxiv; Composition and RNA binding specificity of metazoan RNase MRP; Authors - Yuan Liu