RPP30 Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00472-1

  Sandwich ELISA standard curve of MP00472-1, Human RPP30 Recombinant Matched Antibody Pair - PBS only. 83481-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8600. 83481-5-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

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  Sample test of MP00472-1

  HEK-293 and U-251 cell lysate were measured. The human RPP30 concentration of detected samples was determined to be 194.20 ng/mL (based on a 1.2 mg/mL extract load) in HEK-293 cell lysate and 139.41 ng/mL (based on a 3.3 mg/mL extract load) in U-251 cell lysate.

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  Cytometric bead array standard curve of MP00472-1

  Cytometric bead array standard curve of MP00472-1, RPP30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83481-2-PBS. Detection antibody: 83481-5-PBS. Standard: Ag8600. Range: 0.78-100 ng/mL.

RPP30 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83481-2-PBS

Clone No.240468F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

RNase P subunit 2, RNaseP protein p30, RNASEP2, TSG15

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Product Information

83481-2-PBS targets RPP30 as part of a matched antibody pair:

MP00472-1: 83481-2-PBS capture and 83481-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8600

  Product name: Recombinant human RPP30 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-268 aa of BC006991

  Sequence: MAVFADLDLRAGSDLKALRGLVETAAHLGYSVVAINHIVDFKEKKQEIEKPVAVSELFTTLPIVQGKSRPIKILTRLTIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCITVTEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISSAAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTAFGIISTVKKPRPSEGDEDCLPASKKAKCEG

• Full Name: ribonuclease P/MRP 30kDa subunit
• Calculated Molecular Weight: 268 aa, 29 kDa
• GenBank Accession Number: BC006991
• Gene Symbol: RPP30
• Gene ID (NCBI): 10556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78346
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.