RPP38 Polyclonal antibody

RPP38 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12874-1-AP

Synonyms

RNaseP protein p38, RP11 455B2.5, RPP38

---

Validation Data Gallery

• 

  WB analysis of rat kidney using 12874-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12874-1-AP (RPP38 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12874-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12874-1-AP (RPP38 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12874-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12874-1-AP (RPP38 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12874-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12874-1-AP (RPP38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12874-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12874-1-AP (RPP38 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: rat kidney tissue, HeLa cells, rat liver tissue, mouse liver tissue, human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12874-1-AP targets RPP38 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPP38 fusion protein Ag3552
• Full Name: ribonuclease P/MRP 38kDa subunit
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC029494
• Gene Symbol: RPP38
• Gene ID (NCBI): 10557
• RRID: AB_2285405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Drug Des Devel Ther; Effects of Uremic Clearance Granules on p38 MAPK/NF-κB Signaling Pathway, Microbial and Metabolic Profiles in End-Stage Renal Disease Rats Receiving Peritoneal Dialysis; Authors - Xiaosheng Li
human	WB	Eur J Pharmacol; Tumoral EIF4EBP1 regulates the crosstalk between tumor-associated macrophages and tumor cells in MRTK; Authors - Xin Wu