RPRD1B Polyclonal antibody

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  WB analysis of HeLa using 24542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24542-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 24542-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24542-1-AP (RPRD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

RPRD1B Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24542-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C20orf77, CREPT, RPRD1B

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

24542-1-AP targets RPRD1B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21578

  Product name: Recombinant human RPRD1B protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 79-181 aa of BC033629

  Sequence: REFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELI

• Full Name: regulation of nuclear pre-mRNA domain containing 1B
• Calculated Molecular Weight: 326 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC033629
• Gene Symbol: RPRD1B
• Gene ID (NCBI): 58490
• RRID: AB_2879597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQG5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	bioRxiv; Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PINK1-deficient Human Dopamine Neurons; Authors - Hu Wang ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (05-09-2025)

Works well in western blot (major band between 30 and 42 KDa). Tested on nuclear cell extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat

RPRD1B Antibody Western Blot validation (1:1000 dilution) in Jurkat (Cat no:24542-1-AP)