RPS10 Polyclonal antibody

• 

  WB analysis of Raji using 14894-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14894-1-AP (RPS10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14894-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14894-1-AP (RPS10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 14894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14894-1-AP (RPS10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lymphoma using 14894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14894-1-AP (RPS10 antibody) at dilution of 1:100 (under 40x lens).

RPS10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14894-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ribosomal protein S10, Small ribosomal subunit protein eS10

---

Tested Applications

• Positive WB detected in: Raji cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

14894-1-AP targets RPS10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6683

  Product name: Recombinant human RPS10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-165 aa of BC001032

  Sequence: MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

• Full Name: ribosomal protein S10
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC001032
• Gene Symbol: RPS10
• Gene ID (NCBI): 6204
• RRID: AB_2182493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46783
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS10 (ribosomal protein S10) encodes a ribosomal protein that is a component of the small 40S subunit of the ribosome. RPS10 is essential for cell proliferation and methylation. In nucleoli, RPS10 interacts with nucleophosmin/B23 and is predominantly concentrated in the granular component region, which is required for ribosome assembly (PMID: 20159986, 23723321). Human RPS10 protein consists of 165 amino acids, with a molecular weight of approximately 18.5 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
human	WB	PNAS Nexus; Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability; Authors - Tejinder Pal Khaket
mouse	WB	EMBO Rep; miR-132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity.; Authors - James P Hewitson

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Katie (Verified Customer) (05-23-2022)

Works well for western blot in T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells