RPS10 Monoclonal antibody

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• RPS10 Monoclonal antibody (67609-1-Ig)

• KD/KO Validated

RPS10 Monoclonal Antibody for WB, ELISA

Cat No. 67609-1-Ig

Clone No.1G1E1

Reviews (1)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

1G1E1, ribosomal protein S10, Small ribosomal subunit protein eS10

• 

  WB analysis using 67609-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67609-1-Ig (RPS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 67609-1-Ig

  WB result of RPS10 antibody (67609-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS10 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67609-1-Ig targets RPS10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7018

  Product name: Recombinant human RPS10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-165 aa of BC001032

  Sequence: MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

• Full Name: ribosomal protein S10
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC001032
• Gene Symbol: RPS10
• Gene ID (NCBI): 6204
• RRID: AB_2882815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P46783
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS10 (ribosomal protein S10) encodes a ribosomal protein that is a component of the small 40S subunit of the ribosome. RPS10 is essential for cell proliferation and methylation. In nucleoli, RPS10 interacts with nucleophosmin/B23 and is predominantly concentrated in the granular component region, which is required for ribosome assembly (PMID: 20159986, 23723321). Human RPS10 protein consists of 165 amino acids, with a molecular weight of approximately 18.5 kDa.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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H (Verified Customer) (07-09-2020)

This antibody showed only one band, MW of which corresponded with RPS10.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T cells

RPS10 Antibody Western Blot validation (1:2000 dilution) in HEK293T cells (Cat no:67609-1-Ig)