Tested Applications
| Positive WB detected in | HEK-293 cells, MDA-MB-453s cells, ROS1728 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15942-1-AP targets RPS11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8346 Product name: Recombinant human RPS11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-158 aa of BC007945 Sequence: MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKMKMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF Predict reactive species |
| Full Name | ribosomal protein S11 |
| Calculated Molecular Weight | 158 aa, 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC007945 |
| Gene Symbol | RPS11 |
| Gene ID (NCBI) | 6205 |
| RRID | AB_3085476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62280 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPS11 antibody 15942-1-AP | Download protocol |
| WB protocol for RPS11 antibody 15942-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS11 Polyclonal antibody (Cat# 15942-1-AP) has 1 citation to date. It was used in a study published in Journal for ImmunoTherapy of Cancer (Impact Factor 11.7), investigating how UDP-glucuronate decarboxylase 1 promotes tumor immune evasion by accelerating KMT2D loss in hepatocellular carcinoma. The antibody was applied in Western blot and immunoprecipitation experiments using human and mouse samples. The research falls within the fields of oncology, immune evasion mechanisms, and epigenetic regulation in liver cancer.
| Species | Application | Title |
|---|---|---|
J Immunother Cancer UDP-glucuronate decarboxylase 1 promotes tumor immune evasion by accelerating KMT2D loss in hepatocellular carcinoma |
