RPS12 Monoclonal antibody, PBS Only

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  WB analysis using 67683-1-Ig (same clone as 67683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67683-1-Ig (same clone as 67683-1-PBS)

  WB result of RPS12 antibody (67683-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS12 transfected HepG2 cells. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 67683-1-Ig (same clone as 67683-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67683-1-Ig (same clone as 67683-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67683-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RPS12 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67683-1-PBS

Clone No.1A3D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1A3D1, 40S ribosomal protein S12, ribosomal protein S12, Small ribosomal subunit protein eS12

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Product Information

67683-1-PBS targets RPS12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9649

  Product name: Recombinant human RPS12 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-132 aa of BC017321

  Sequence: MAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVDDNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK

• Full Name: ribosomal protein S12
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC017321
• Gene Symbol: RPS12
• Gene ID (NCBI): 6206
• RRID: AB_2882876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P25398
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.