RPS14 Monoclonal antibody, PBS Only

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  WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67566-1-Ig (same clone as 67566-1-PBS)

  WB result of RPS14 antibody (67566-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPS14 transfected HeLa cells. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67566-1-Ig (same clone as 67566-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67566-1-Ig (RPS14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 67566-1-Ig (same clone as 67566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67566-1-Ig (RPS14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67566-1-Ig (same clone as 67566-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS14 antibody (67566-1-Ig, Clone: 3D5H5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67566-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RPS14 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67566-1-PBS

Clone No.3D5H5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RPS 14, ribosomal protein S14, PRO2640, EMTB, 40S ribosomal protein S14

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Product Information

67566-1-PBS targets RPS14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30032

  Product name: Recombinant human RPS14 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-151 aa of BC003401

  Sequence: MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL

• Full Name: ribosomal protein S14
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC003401
• Gene Symbol: RPS14
• Gene ID (NCBI): 6208
• RRID: AB_2882780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62263
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].