RPS15A Polyclonal antibody

RPS15A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14942-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

40S ribosomal protein S15a, ribosomal protein S15a, RPS15A, S15a

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 14942-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14942-1-AP (RPS15A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 14942-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 14942-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HeLa cells, HepG2 cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14942-1-AP targets RPS15A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6655 Product name: Recombinant human RPS15A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-130 aa of BC001697 Sequence: MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRSDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF Predict reactive species
Full Name	ribosomal protein S15a
Calculated Molecular Weight	15 kDa
Observed Molecular Weight	13-15 kDa
GenBank Accession Number	BC001697
Gene Symbol	RPS15A
Gene ID (NCBI)	6210
RRID	AB_3085446
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62244
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.