RPS17 Polyclonal antibody

RPS17 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16267-1-AP

Synonyms

40S ribosomal protein S17, DBA4, ribosomal protein S17, RPS17, RPS17L1, RPS17L2

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Validation Data Gallery

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  WB analysis using 16267-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16267-1-AP (RPS17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 16267-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16267-1-AP (RPS17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 16267-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16267-1-AP (RPS17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, PC-3 cells, NIH/3T3 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16267-1-AP targets RPS17 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS17 fusion protein Ag9334
• Full Name: ribosomal protein S17
• Calculated Molecular Weight: 135 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC009407
• Gene Symbol: RPS17
• Gene ID (NCBI): 6218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.