Tested Applications
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14796-1-AP targets RPS28 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6505 Product name: Recombinant human RPS28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-69 aa of BC000354 Sequence: MDTSRVQPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRLR Predict reactive species |
| Full Name | ribosomal protein S28 |
| Calculated Molecular Weight | 8 kDa |
| GenBank Accession Number | BC000354 |
| Gene Symbol | RPS28 |
| Gene ID (NCBI) | 6234 |
| RRID | AB_2285281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62857 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPS28 antibody 14796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS28 Polyclonal antibody (Cat# 14796-1-AP) has 5 total citations published in Cancer Research, Cancer Letters, Journal of Orthopaedic Translation, International Journal of Biological Macromolecules, and Clinical and Translational Medicine. Research fields span cancer biology (p53 regulation, oncogenic translation, glioblastoma), ribosome function, orthopedic pathology (heterotopic ossification), and pediatric gastroenterology (necrotising enterocolitis).
| Species | Application | Title |
|---|---|---|
Cancer Lett Fibrillarin-dependent 2'-O-methylation modulates RPS28 ribosome incorporation and oncogenic translation.
| ||
J Orthop Translat Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression. | ||
Int J Biol Macromol Translational hub ribosomal protein S5 promotes glioblastoma progression by affecting translation patterns. | ||
Clin Transl Med Multiomics analyses revealed the roles of the epithelial-mesenchymal transition and novel early candidate diagnostic biomarkers in neonatal necrotising enterocolitis. |
