RPS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPS3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (2)

Cat no : 11990-1-AP

Synonyms

40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3

---

Validation Data Gallery

• 

  WB analysis using 11990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11990-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 11990-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11990-1-AP (RPS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11990-1-AP

  IP result of anti-RPS3 (IP:11990-1-AP, 3ug; Detection:11990-1-AP 1:600) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human ovary tumor using 11990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11990-1-AP (RPS3 antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue, PC-12 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Product Information

11990-1-AP targets RPS3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS3 fusion protein Ag3918
• Full Name: ribosomal protein S3
• Calculated Molecular Weight: 26 aa, 7 kDa
• Observed Molecular Weight: 26.7 kDa
• GenBank Accession Number: BC034149
• Gene Symbol: RPS3
• Gene ID (NCBI): 6188
• RRID: AB_2180758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

Publications

Species	Application	Title
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy
mouse	IF	Nat Commun; Cilia locally synthesize proteins to sustain their ultrastructure and functions.; Authors - Kai Hao
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
pig	IF	J Anim Sci Biotechnol; Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.; Authors - Chunhua Xing
human	WB,IHC	Front Oncol; RPS3 Promotes the Metastasis and Cisplatin Resistance of Adenoid Cystic Carcinoma.; Authors - Xi Rui
human	IF	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a clean band at 27 kd.

• Applications: Western Blot

FH

Huanzhou (Verified Customer) (11-26-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cl-16