RPS3 Polyclonal antibody
RPS3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15198-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, NIH/3T3 cells, mouse colon tissue, COLO 320 cells, HeLa cells, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human ovary tumor tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
15198-1-AP targets RPS3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | yeast |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RPS3 fusion protein Ag7346 |
Full Name | ribosomal protein S3 |
Calculated Molecular Weight | 27 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC003137 |
Gene Symbol | RPS3 |
Gene ID (NCBI) | 6188 |
RRID | AB_2253905 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ribosomal protein S3 is a subunit of 40S ribosomal protein, and has two distinct function. RPS3 acts as a ribosome protein that contributes to the domain of the ribosome where translation is initiated, and involves in repair of UV damage as an endonuclease. Besides binding RNA within the 40S ribosomal subunit, RPS3 participates in transcription, and apoptosis.
Protocols
Product Specific Protocols | |
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WB protocol for RPS3 antibody 15198-1-AP | Download protocol |
IHC protocol for RPS3 antibody 15198-1-AP | Download protocol |
IP protocol for RPS3 antibody 15198-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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