RPS3 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84947-4-RR (same clone as 84947-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84947-4-RR (RPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84947-4-RR (same clone as 84947-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84947-4-RR (RPS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 84947-4-RR (same clone as 84947-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RPS3 antibody (84947-4-RR, Clone: 242504F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84947-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84947-4-RR against Human RPS3 were performed. The affinity constant is below 1 pM.

RPS3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84947-4-PBS

Clone No.242504F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450

---

Product Information

84947-4-PBS targets RPS3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7346

  Product name: Recombinant human RPS3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-243 aa of BC003137

  Sequence: MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA

• Full Name: ribosomal protein S3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC003137
• Gene Symbol: RPS3
• Gene ID (NCBI): 6188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P23396
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.