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RPS4Y1 Polyclonal antibody, PBS Only (17296-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of DU 145 using 17296-1-AP (same clone as 17296-1-PBS)
DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
WB analysis of A2780 using 17296-1-AP (same clone as 17296-1-PBS)
A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
WB analysis of SKOV-3 using 17296-1-AP (same clone as 17296-1-PBS)
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 17296-1-AP (same clone as 17296-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
WB analysis of DU 145 using 17296-1-AP (same clone as 17296-1-PBS)
DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
WB analysis of A2780 using 17296-1-AP (same clone as 17296-1-PBS)
A2780 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
WB analysis of mouse ovary using 17296-1-AP (same clone as 17296-1-PBS)
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 17296-1-AP (same clone as 17296-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 17296-1-AP (same clone as 17296-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17296-1-AP (RPS4Y1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 17296-1-AP (same clone as 17296-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS4Y1 antibody (17296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

RPS4Y1 Polyclonal antibody, PBS Only

Name: RPS4Y1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 17296-1-PBS

RPS4Y1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 17296-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

17296-1-PBS targets RPS4Y1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11176 Product name: Recombinant human RPS4Y1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-263 aa of BC010286 Sequence: KKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIVFLRNRLKYALTGDEVKKICMQRFIKIDGKVRVDVTYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITVEEAKYKLCKVRKITVGVKGIPHLVTHDARTIRYPDPVIKVNDTVQIDLGTGKIINFIKFDTGNLCMVIGGANLGRVGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGNGNKPWISLPRGKGIRLTVAEERDKRLATKQSSG Predict reactive species
Full Name	ribosomal protein S4, Y-linked 1
Calculated Molecular Weight	263 aa, 29 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC010286
Gene Symbol	RPS4Y1
Gene ID (NCBI)	6192
RRID	AB_2180889
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22090
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RPS4Y1, a Y-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). RPS4Y maps to a 90-kb segment that has been implicated in Turner syndrome. As a homolog with RPS4X, the antibody can recognize endogenous level of RPS4Y and RPS4X, at 29-32kDa.