Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16964-1-AP targets RPS5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, c.elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10686 Product name: Recombinant human RPS5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC018151 Sequence: MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR Predict reactive species |
| Full Name | ribosomal protein S5 |
| Calculated Molecular Weight | 204 aa, 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC018151 |
| Gene Symbol | RPS5 |
| Gene ID (NCBI) | 6193 |
| RRID | AB_2180894 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46782 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS5 antibody 16964-1-AP | Download protocol |
| IHC protocol for RPS5 antibody 16964-1-AP | Download protocol |
| WB protocol for RPS5 antibody 16964-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RPS5 antibody (Cat# 16964-1-AP) has 10 total citations published in journals including Cancer Cell, Nature Cell Biology, Nature Communications, Acta Pharmaceutica Sinica B, Nature Structural & Molecular Biology, International Journal of Biological Macromolecules, Journal of Cell Biology, Journal of Biochemical and Molecular Toxicology, Biophysical Reports, and eLife. Research fields span prostate cancer, ribophagy, proteasome interactions, breast cancer metabolism, mRNA translation, glioblastoma progression, ribosome biogenesis, pulmonary inflammation, and nuclear import.
| Species | Application | Title |
|---|---|---|
Nat Commun In-situ cross-linking mass spectrometry reveals compartment-specific proteasomal interactions and structural heterogeneity. | ||
Acta Pharm Sin B (+)-Miliusol suppresses the Warburg effect and induces regulated cell death in triple-negative breast cancer through targeting EIF3D and remodeling cancer metabolism. | ||
Nat Struct Mol Biol Rps26 directs mRNA-specific translation by recognition of Kozak sequence elements. | ||
Int J Biol Macromol Translational hub ribosomal protein S5 promotes glioblastoma progression by affecting translation patterns.
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