RPS6KA1 Polyclonal antibody

RPS6KA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16463-1-AP

Synonyms

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

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Validation Data Gallery

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  WB analysis of K-562 using 16463-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16463-1-AP (RPS6KA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16463-1-AP

  IP result of anti-RPS6KA1 (IP:16463-1-AP, 4ug; Detection:16463-1-AP 1:300) with K-562 cells lysate 2800ug.

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  IHC staining of human liver cancer using 16463-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16463-1-AP (RPS6KA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16463-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16463-1-AP (RPS6KA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

16463-1-AP targets RPS6KA1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS6KA1 fusion protein Ag9537
• Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Calculated Molecular Weight: 735 aa, 83 kDa
• Observed Molecular Weight: 83-90, 73 kDa
• GenBank Accession Number: BC014966
• Gene Symbol: RPS6KA1
• Gene ID (NCBI): 6195
• RRID: AB_2878262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation (PMID: 16458888, 30813401, 17259979, 10679322)

Publications

Species	Application	Title
human	WB	Cell Death Differ; β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.; Authors - Jia Ma
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	Oncogenesis; GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.; Authors - Chenggang Zhao
rat	WB	J Cell Physiol; Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p.; Authors - Qing-Ping Yao
human	WB	ACS Omega; Proteomic Changes in the Monolayer and Spheroid Melanoma Cell Models of Acquired Resistance to BRAF and MEK1/2 Inhibitors.; Authors - Ramon Martinez
human	WB	Cell Death Dis; Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration; Authors - Yunfeng Hu