MSK1 Polyclonal antibody

• 

  IHC staining of human lung cancer using 15977-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15977-1-AP (MSK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15977-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15977-1-AP (MSK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 15977-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MSK1 antibody (15977-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

MSK1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 15977-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

RPS6KA5, EC:2.7.11.1, MSPK1, Nuclear mitogen- and stress-activated protein kinase 1, Ribosomal protein S6 kinase alpha-5

---

Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15977-1-AP targets MSK1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSK1 fusion protein Ag8765
• Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 5
• Calculated Molecular Weight: 549aa,62 kDa; 802aa,90 kDa
• GenBank Accession Number: BC017187
• Gene Symbol: MSK1
• Gene ID (NCBI): 9252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75582
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Genes Dev; Regulation of DNA demethylation by the XPC DNA repair complex in somatic and pluripotent stem cells.; Authors - Jaclyn J Ho
		Proc Natl Acad Sci U S A; Functional and mechanistic studies of XPC DNA-repair complex as transcriptional coactivator in embryonic stem cells.; Authors - Claudia Cattoglio