MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15268-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 15268-1-AP
IP result of anti-S6K2 (IP:15268-1-AP, 4ug; Detection:15268-1-AP 1:1000) with MCF-7 cells lysate 2800ug.
IP result of anti-S6K2 (IP:15268-1-AP, 4ug; Detection:15268-1-AP 1:1000) with MCF-7 cells lysate 2800ug.
IHC staining of human kidney using 15268-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15268-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 15268-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15268-1-AP (S6K2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15268-1-AP (S6K2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
RPS6KB2(ribosomal protein S6 kinase beta-2) is also named as 70 kDa ribosomal protein S6 kinase 2(P70S6K2),Serine/threonine-protein kinase 14B(STK14B) and belongs to the protein kinase superfamily. It is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. The activated enzyme phosphorylates several serine residues in the C terminus of ribosomal protein S6(PMID:9878560).
MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15268-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human kidney using 15268-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15268-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of MCF-7 using 15268-1-AP
IP result of anti-S6K2 (IP:15268-1-AP, 4ug; Detection:15268-1-AP 1:1000) with MCF-7 cells lysate 2800ug.
IF/ICC Figures
IF Staining of MCF-7 using 15268-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15268-1-AP (S6K2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RPS6KB2 Polyclonal antibody
Catalog Number
15268-1-AP
Citations
2
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
