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RPS6KB2 Polyclonal antibody

RPS6KB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

KLS, P70 beta, P70 beta 1, P70 beta 2, p70 ribosomal S6 kinase beta, p70 S6 kinase beta, p70 S6K 2, p70 S6K beta, p70 S6KB, p70(S6K) beta, P70S6K2, p70S6Kb, RPS6KB2, S6 kinase related kinase, S6K beta, S6K beta 2, S6K beta2, S6K2, SRK, STK14B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Freight/Packing: -

Quantity

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What's the best dilution for RPS6KB2 in ?

Tested Applications

Positive WB detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26194-1-AP targets RPS6KB2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23800

Product name: Recombinant human RPS6KB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-51 aa of BC000094

Sequence: MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELT

Predict reactive species
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 2
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC000094
Gene Symbol RPS6KB2
Gene ID (NCBI) 6199
RRIDAB_2880419
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBS0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPS6KB2 antibody 26194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The RPS6KB2 Polyclonal antibody (Cat# 26194-1-AP) has 3 total citations. These appear in bioRxiv, eLife, and Molecular Therapy Nucleic Acids. The cited research spans oncology (lncRNA CyKILRb activating the PI3K/AKT axis in cancer), translational biology (eIF2A-mediated translational control in HeLa cells), and molecular therapy involving ceRNA mechanisms in tumor suppression. All citations used the antibody for Western blot applications in human samples.

SpeciesApplicationTitle
humanWB

bioRxiv

The long non-coding RNA, CyKILRb, augments oncogenic phenotypes via induction of PIK3R2 and activation of the PI (3) K/AKT axis.

Authors - Xiujie Xie
humanWB

Elife

Human eIF2A has a minimal role in translation initiation and in uORF-mediated translational control in HeLa cells

Authors - Mykola Roiuk

Mol Ther Nucleic Acids

The lncRNA, CyKILRb acts as a ceRNA for tumor suppressive microRNA to augment oncogenic phenotype via induction of PIK3R2/PI(3)K/AKT axis.

Authors - Xiujie Xie
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