• Featured Product
  • KD/KO Validated

RPS7 Polyclonal antibody

RPS7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14491-1-AP

Synonyms

40S ribosomal protein S7, DBA8, ribosomal protein S7, RPS7



Tested Applications

Positive WB detected inNIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14491-1-AP targets RPS7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS7 fusion protein Ag5892
Full Name ribosomal protein S7
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC061901
Gene Symbol RPS7
Gene ID (NCBI) 6201
RRIDAB_11232411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S7(RPS7) belongs to the RPs, which is structural compoent of the ribosome invilved in protein synthesis. It interacts with and inhibit mdm2 mediated p53 degradation function. Also RPS7 has a essential role in early development. RPS7 involves in the maturation of ribosomal RNAs in the large or the small ribosomal subunit production pathway

Protocols

Product Specific Protocols
WB protocol for RPS7 antibody 14491-1-APDownload protocol
IHC protocol for RPS7 antibody 14491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Chem Biol

Identifying the functional contribution of the defatty-acylase activity of SIRT6.

Authors - Xiaoyu Zhang
humanWB

Sci Rep

Stabilization of SIRT7 deacetylase by viral oncoprotein HBx leads to inhibition of growth restrictive RPS7 gene and facilitates cellular transformation.

Authors - Vijaya Pandey
humanWB

Exp Cell Res

Combination of DESI2 and endostatin gene therapy significantly improves antitumor efficacy by accumulating DNA lesions, inducing apoptosis and inhibiting angiogenesis.

Authors - Huaying Yan
humanWB

Cell Death Dis

SMYD2 promotes tumorigenesis and metastasis of lung adenocarcinoma through RPS7.

Authors - Lei Wu
mouseWB,IHC,IF

Biomed Res Int

Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury

Authors - Hao Zhang
  • KD Validated
humanWB

Cell

Human hematopoietic stem cell vulnerability to ferroptosis

Authors - Jiawei Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (07-09-2020)

This antibody has so many non-specific bands. Considering the fact that ribosomal proteins are so abundant, having these many non-specific bands is not usual for an r-protein antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
RPS7 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:14491-1-AP)