RPS8 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• RPS8 Polyclonal antibody (18228-1-AP)

RPS8 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 18228-1-AP Copied!

Publications (7)

Reviews (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Small ribosomal subunit protein eS8, ribosomal protein S8, OK/SW-cl.83, 40S ribosomal protein S8

• 

  WB analysis using 18228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SGC-7901 using 18228-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 18228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 18228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 18228-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 18228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 18228-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A375 using 18228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A375 using 18228-1-AP

  Immunofluorescent analysis of A375 cells using 18228-1-AP (RPS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for RPS8 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, SGC-7901 cells, PC-3 cells, mouse pancreas tissue, A375 cells
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18228-1-AP targets RPS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12313

  Product name: Recombinant human RPS8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-208 aa of BC070875

  Sequence: MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK

• Full Name: ribosomal protein S8
• Calculated Molecular Weight: 208 aa, 24 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC070875
• Gene Symbol: RPS8
• Gene ID (NCBI): 6202
• RRID: AB_10666168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62241
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPS8 Polyclonal antibody (Cat# 18228-1-AP) has 7 citations published in Nature Cell Biology, Cancer Biology & Medicine, Developmental Cell, Molecular & Cellular Proteomics, International Journal of Molecular Sciences, Oncology Reports, and Laboratory Investigation. Research fields span extracellular vesicles, lung adenocarcinoma prognosis, autophagy and spermiogenesis, exosome proteomics in breast cancer, RNA-protein complex isolation, hepatocellular carcinoma biomarkers, and pancreatic cancer survival.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Cell Biol; Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties; Authors - Dennis K Jeppesen
human	IHC	Cancer Biol Med; A novel recurrence-associated metabolic prognostic model for risk stratification and therapeutic response prediction in patients with stage I lung adenocarcinoma.; Authors - Chengming Liu
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
human	WB	Int J Mol Sci; Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1.; Authors - Yangchao Dong
human	IHC	Oncol Rep; Identification of 40S ribosomal protein S8 as a novel biomarker for alcohol‑associated hepatocellular carcinoma using weighted gene co‑expression network analysis.; Authors - Ningrui Bi

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

One reviewer rated this product 4 out of 5 stars for Western Blot. They reported successful use at a 1:1000 dilution on both HeLa cells and C. elegans nematodes, using lot 00013018. Overall, the feedback is positive, confirming reliable performance across species at the recommended dilution.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

Anita (Verified Customer) (10-30-2020)

Works at 1:1000 dilution for HeLa cells and C. elegans nematodes

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans and HeLa cells

RPS8 Antibody Western Blot validation (1:1000 dilution) in C. elegans and HeLa cells (Cat no:18228-1-AP)