RPS8 Polyclonal antibody

RPS8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 18228-1-AP

Synonyms

Small ribosomal subunit protein eS8, ribosomal protein S8, OK/SW-cl.83, 40S ribosomal protein S8

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Validation Data Gallery

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  WB analysis using 18228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 18228-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 18228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18228-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 18228-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 18228-1-AP

  Immunofluorescent analysis of A375 cells using 18228-1-AP (RPS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, SGC-7901 cells, PC-3 cells, mouse pancreas tissue, A375 cells
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below

Product Information

18228-1-AP targets RPS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS8 fusion protein Ag12313
• Full Name: ribosomal protein S8
• Calculated Molecular Weight: 208 aa, 24 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC070875
• Gene Symbol: RPS8
• Gene ID (NCBI): 6202
• RRID: AB_10666168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	IHC	Cancer Biol Med; A novel recurrence-associated metabolic prognostic model for risk stratification and therapeutic response prediction in patients with stage I lung adenocarcinoma.; Authors - Chengming Liu
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
human	IHC	Lab Invest; Proteins associated with pancreatic cancer survival in patients with resectable pancreatic ductal adenocarcinoma.; Authors - Ru Chen
human	IHC	Oncol Rep; Identification of 40S ribosomal protein S8 as a novel biomarker for alcohol‑associated hepatocellular carcinoma using weighted gene co‑expression network analysis.; Authors - Ningrui Bi
human	WB	Int J Mol Sci; Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1.; Authors - Yangchao Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anita (Verified Customer) (10-30-2020)

Works at 1:1000 dilution for HeLa cells and C. elegans nematodes

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans and HeLa cells

RPS8 Antibody Western Blot validation (1:1000 dilution) in C. elegans and HeLa cells (Cat no:18228-1-AP)