RPS9 Polyclonal antibody

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  WB analysis of mouse uterus using 18215-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 18215-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 18215-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18215-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 18215-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPS9 antibody (18215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

RPS9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18215-1-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

40S ribosomal protein S9, ribosomal protein S9, Small ribosomal subunit protein uS4

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Tested Applications

• Positive WB detected in: mouse uterus tissue, HeLa cells, mouse ovary tissue
• Positive IHC detected in: human lung tissue, human ovary tissue, human skin tissue, human testis tissue, mouse lung tissue, mouse pancreas tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

18215-1-AP targets RPS9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12945

  Product name: Recombinant human RPS9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-194 aa of BC000802

  Sequence: MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED

• Full Name: ribosomal protein S9
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000802
• Gene Symbol: RPS9
• Gene ID (NCBI): 6203
• RRID: AB_2182390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46781
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Proc Natl Acad Sci U S A; Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.; Authors - Elizabeth I Vink ; KD Validated
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	Sci Rep; Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.; Authors - Haiping Tang
human	WB	Front Immunol; Gradient Boosting Machine Learning Model for Defective Endometrial Receptivity Prediction by Macrophage-Endometrium Interaction Modules.; Authors - Bohan Li
human	WB	Genes (Basel); Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.; Authors - Meijuan Zou ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Single, strong band as predicted size 25kDa

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart