RPS9 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• RPS9 Polyclonal antibody (18215-1-AP)

• Featured Product
• KD/KO Validated

RPS9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18215-1-AP Copied!

Publications (11)

Reviews (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

40S ribosomal protein S9, ribosomal protein S9, Small ribosomal subunit protein uS4

• 

  WB analysis of mouse uterus using 18215-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 18215-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 18215-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 18215-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18215-1-AP (RPS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse stomach using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18215-1-AP (RPS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 18215-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18215-1-AP (RPS9 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 18215-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RPS9 antibody (18215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for RPS9 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: mouse uterus tissue, HeLa cells, mouse ovary tissue
• Positive IHC detected in: human lung tissue, human ovary tissue, human skin tissue, human testis tissue, mouse lung tissue, mouse pancreas tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18215-1-AP targets RPS9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12945

  Product name: Recombinant human RPS9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-194 aa of BC000802

  Sequence: MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED

• Full Name: ribosomal protein S9
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000802
• Gene Symbol: RPS9
• Gene ID (NCBI): 6203
• RRID: AB_2182390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46781
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPS9 Polyclonal antibody (Cat# 18215-1-AP) has 11 total citations published in Molecular Psychiatry, Journal of Translational Medicine, PNAS, Developmental Cell, Cell Reports, Frontiers in Immunology, Scientific Reports, Journal of Cancer, and Genes (Basel). Associated research fields include neurodevelopmental disorders, embryonic development, nuclear condensate biology, spermiogenesis, cellular senescence, endometrial immunology, and oncology (glioblastoma, osteosarcoma, NSCLC).

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Mol Psychiatry; Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome; Authors - Federica Giona
mouse	WB	J Transl Med; Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice.; Authors - Xin Li
human	WB	Proc Natl Acad Sci U S A; Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.; Authors - Elizabeth I Vink ; KD Validated
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	IF	Cell Rep; Senescent cells form nuclear foci that contain the 26S proteasome; Authors - Tomohiro Iriki

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for 18215-1-AP is from Wei Feng (January 2020), who used the antibody for Western Blot on mouse heart tissue (lot 00009939). They reported a single, strong band at the predicted size of 25 kDa and gave it a perfect 5-star rating. Overall, the feedback is positive, confirming clear and specific detection in the tested application.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

Wei (Verified Customer) (01-30-2020)

Single, strong band as predicted size 25kDa

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart