RPUSD2 Polyclonal antibody

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  WB analysis of HeLa using 25707-1-AP

  WB result of RPUSD2 antibody (25707-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPUSD2 transfected HeLa cells.

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  WB analysis using 25707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25707-1-AP (RPUSD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25707-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25707-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25707-1-AP (RPUSD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RPUSD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25707-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RNA pseudouridylate synthase domain-containing protein 2, Pseudouridylate synthase RPUSD2, EC:5.4.99.-, C18B11, C15orf19

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25707-1-AP targets RPUSD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPUSD2 fusion protein Ag22452
• Full Name: RNA pseudouridylate synthase domain containing 2
• Calculated Molecular Weight: 545 aa, 61 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC016967
• Gene Symbol: RPUSD2
• Gene ID (NCBI): 27079
• RRID: AB_2880202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IZ73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.