RRAGB Polyclonal antibody

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RRAGB Polyclonal Antibody for WB, IP, ELISA

Cat No. 13023-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

EC:3.6.5.-, Rag B, RagB, Ras related GTP binding B, Ras related GTP binding protein B

• 

  WB analysis using 13023-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 13023-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 13023-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 13023-1-AP

  IP result of anti-RRAGB (IP:13023-1-AP, 4ug; Detection:13023-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, human testis tissue, human brain tissue, HeLa cells, Jurkat cells, fetal human brain tissue, mouse testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13023-1-AP targets RRAGB in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4116

  Product name: Recombinant human RRAGB protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-346 aa of BC034726

  Sequence: MEESDSEKTTEKENLGPRMDPPLGEPEGSLGWVLPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR

• Full Name: Ras-related GTP binding B
• Calculated Molecular Weight: 346 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC034726
• Gene Symbol: RRAGB
• Gene ID (NCBI): 10325
• RRID: AB_2180064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5VZM2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-RRAGB antibody (Cat# 13023-1-AP) has 9 citations across journals including Molecular Cancer, Cell Metabolism, Nature Chemical Biology, Journal of Clinical Investigation, Cell Death & Disease, Cell Signaling, PLoS One, Biochemical and Biophysical Research Communications, and Physiological Reports. Research fields span mTORC1 signaling, cancer (colorectal cancer, glioblastoma), metabolism (pyruvate/leucine catabolism), cardiomyopathy, diabetic kidney disease, and neurochemistry.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,RIP,IHC	Mol Cancer; circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.; Authors - Xiaomin Li
human	WB,IP	Cell Metab; Pyruvate metabolism enzyme DLAT promotes tumorigenesis by suppressing leucine catabolism; Authors - Ning Wang
mouse	WB	Nat Chem Biol; Designing chemigenetic DNA nanotrap for norepinephrine dynamic imaging in organelles.; Authors - Yilin Chen
mouse	WB,IF	J Clin Invest; YAP plays a crucial role in the development of cardiomyopathy in lysosomal storage diseases.; Authors - Shohei Ikeda ; KO Validated
mouse,human	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li
mouse	WB	Cell Signal; CircMRP4 orchestrates podocytes injury via the miR-499-5p/RRAGB/mTORC1 axis in diabetic kidney disease; Authors - Shujun Deng