RRAGB Polyclonal antibody

• Featured Product
• KD/KO Validated

RRAGB Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13023-1-AP

Synonyms

Rag B, RAGB, Ras related GTP binding B, RRAGB

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Validation Data Gallery

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  WB analysis of human testis using 13023-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13023-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13023-1-AP (RRAGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13023-1-AP

  IP result of anti-RRAGB (IP:13023-1-AP, 4ug; Detection:13023-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human brain using 13023-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13023-1-AP (RRAGB Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human testis tissue, human brain tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

13023-1-AP targets RRAGB in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RRAGB fusion protein Ag4116
• Full Name: Ras-related GTP binding B
• Calculated Molecular Weight: 346 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC034726
• Gene Symbol: RRAGB
• Gene ID (NCBI): 10325
• RRID: AB_2180064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC,RIP	Mol Cancer; circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.; Authors - Xiaomin Li
mouse	WB, IF	J Clin Invest; YAP plays a crucial role in the development of cardiomyopathy in lysosomal storage diseases.; Authors - Shohei Ikeda ; KO Validated
mouse	WB	PLoS One; Ursolic Acid Inhibits Leucine-Stimulated mTORC1 Signaling by Suppressing mTOR Localization to Lysosome.; Authors - Xiang Ou
	WB	Biochem Biophys Res Commun; RRAGB-mediated suppression of PI3K/AKT exerts anti-cancer role in glioblastoma; Authors - Qinggang Li