RRBP1 Recombinant monoclonal antibody, PBS Only

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  IF Staining of HeLa using 82890-1-RR (same clone as 82890-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 82890-1-RR (same clone as 82890-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 82890-1-RR (same clone as 82890-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

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  FC experiment of U-2 OS using 82890-1-RR (same clone as 82890-1-PBS)

  (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-1-RR, Clone:230159G6) and Alexa Fluor™ Plus 647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82890-1-RR (same clone as 82890-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-1-RR, Clone:230159G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82890-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82890-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82890-1-RR against Human RRBP1 were performed. The affinity constant is below 1 pM.

RRBP1 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82890-1-PBS

Clone No.230159G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

180 kDa ribosome receptor homolog, 230159G6, ES/130 related protein, ES/130-related protein, Ribosome-binding protein 1

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Product Information

82890-1-PBS targets RRBP1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16648

  Product name: Recombinant human RRBP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1061-1410 aa of BC128577

  Sequence: AQTMEALLALLPELSVLAQQNYTEWLQDLKEKGPTLLKHPPAPAEPSSDLASKLREAEETQSTLQAECDQYRSILAETEGMLRDLQKSVEEEEQVWRAKVGAAEEELQKSRVTVKHLEEIVEKLKGELESSDQVREHTLHLEAELEKHMAAASAECQNYAKEVAGLRQLLLESQSQLDAAKSEAQKQSDELALVRQQLSEMKSHVEDGDIAGAPASSPEAPPAEQDPVQLKTQLEWTEAILEDEQTQRQKLTAEFEEAQTSACRLQEELEKLRTAGPLESSETEEASQLKERLEKEKKLTSDLGRAATRLQELLKTTQEQLAREKDTVKKLQEQLEKAEDGSSSKEGTSV

• Full Name: ribosome binding protein 1 homolog 180kDa (dog)
• Calculated Molecular Weight: 1410 aa, 152 kDa
• Observed Molecular Weight: 108 kDa, 152 kDa
• GenBank Accession Number: BC128577
• Gene Symbol: RRBP1
• Gene ID (NCBI): 6238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P2E9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)