RRM2 Monoclonal antibody

RRM2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2A9A7

Cat no : 67006-1-Ig

Synonyms

RR2M, RR2, R2, EC:1.17.4.1, 2A9A7

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Validation Data Gallery

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  WB analysis using 67006-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67006-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67006-1-Ig (RRM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 67006-1-Ig

  IP result of anti-RRM2 (IP:67006-1-Ig, 4ug; Detection:67006-1-Ig 1:2000) with HEK-293 cells lysate 1470 ug.

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  IHC staining of human skin cancer using 67006-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67006-1-Ig (RRM2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67006-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM2 antibody (67006-1-Ig, Clone: 2A9A7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, A431 cells, Jurkat cells, K-562 cells, THP-1 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

67006-1-Ig targets RRM2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RRM2 fusion protein Ag28664
• Full Name: ribonucleotide reductase M2 polypeptide
• Calculated Molecular Weight: 389 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC030154
• Gene Symbol: RRM2
• Gene ID (NCBI): 6241
• RRID: AB_2882323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].

Publications

Species	Application	Title
human	WB	Neoplasma; The SNHG16/miR-30a axis promotes breast cancer cell proliferation and invasion by regulating RRM2.; Authors - S M Du
human	WB,CoIP	Chem Biol Interact; Therapeutic Targeting of Thioredoxin Reductase 1 Causes Ferroptosis while Potentiating Anti-PD-1 Efficacy in Head and Neck Cancer; Authors - Ming-Shou Hsieh