Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, A431 cells, Jurkat cells, K-562 cells, THP-1 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67006-1-Ig targets RRM2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28664 Product name: Recombinant human RRM2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-389 aa of BC030154 Sequence: MLSLRVPLAPITDPQQLQLSPLKGLSLVDKENTPPALSGTRVLASKTARRIFQEPTEPKTKAAAPGVEDEPLLRENPRRFVIFPIEYHDIWQMYKKAEASFWTAEEVDLSKDIQHWESLKPEERYFISHVLAFFAASDGIVNENLVERFSQEVQITEARCFYGFQIAMENIHSEMYSLLIDTYIKDPKEREFLFNAIETMPCVKKKADWALRWIGDKEATYGERVVAFAAVEGIFFSGSFASIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFKHLVHKPSEERVREIIINAVRIEQEFLTEALPVKLIGMNCTLMKQYIEFVADRLMLELGFSKVFRVENPFDFMENISLEGKTNFFEKRVGEYQRMGVMSSPTENSFTLDADF Predict reactive species |
| Full Name | ribonucleotide reductase M2 polypeptide |
| Calculated Molecular Weight | 389 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC030154 |
| Gene Symbol | RRM2 |
| Gene ID (NCBI) | 6241 |
| RRID | AB_2882323 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P31350 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RRM2 antibody 67006-1-Ig | Download protocol |
| IHC protocol for RRM2 antibody 67006-1-Ig | Download protocol |
| IP protocol for RRM2 antibody 67006-1-Ig | Download protocol |
| WB protocol for RRM2 antibody 67006-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RRM2 antibody (Cat# 67006-1-Ig) has 7 total citations published in Frontiers in Pharmacology, Chemical & Biological Interactions, Molecular Cell Biochemistry, iScience, World Journal of Gastrointestinal Oncology, Neoplasma, and Histology and Histopathology. Research fields span ferroptosis regulation, cancer biology (ovarian, head and neck, hepatocellular, pancreatic, breast, and cervical cancers), drug resistance mechanisms, and post-transcriptional mRNA stabilization. Applications include WB, CoIP, and IF in human and mouse models.
| Species | Application | Title |
|---|---|---|
Front Pharmacol CircASH1L inhibits ferroptosis and enhances cisplatin resistance by sponging miR-515-5p to regulate cell cycle-related CDCA7/RRM2 in ovarian cancer cells | ||
Chem Biol Interact Therapeutic Targeting of Thioredoxin Reductase 1 Causes Ferroptosis while Potentiating Anti-PD-1 Efficacy in Head and Neck Cancer | ||
Mol Cell Biochem FOXM1-activated IGF2BP3 promotes cell malignant phenotypes and M2 macrophage polarization in hepatocellular carcinoma by inhibiting ferroptosis via stabilizing RRM2 mRNA in an m6A-dependent manner | ||
iScience RRM2 promotes liver metastasis of pancreatic cancer by stabilizing YBX1 and activating the TGF-beta pathway | ||
World J Gastrointest Oncol DEAD/H-box RNA helicase 10 promotes pancreatic cancer cell proliferation via ribonucleotide reductase M2 | ||
Neoplasma The SNHG16/miR-30a axis promotes breast cancer cell proliferation and invasion by regulating RRM2. |
