RRM2B,p53R2 Polyclonal antibody

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  WB analysis using 18005-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18005-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18005-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 18005-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18005-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 18005-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18005-1-AP (RRM2B,p53R2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RRM2B,p53R2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18005-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RRM2B, TP53-inducible ribonucleotide reductase M2 B, Ribonucleoside-diphosphate reductase subunit M2 B, p53R2, p53-inducible ribonucleotide reductase small subunit 2-like protein

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Tested Applications

• Positive WB detected in: A431 cells, PC-3 cells, MDA-MB-453s cells, HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

18005-1-AP targets RRM2B,p53R2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12499

  Product name: Recombinant human RRM2B,p53R2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC108261

  Sequence: MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDAD

• Full Name: ribonucleotide reductase M2 B (TP53 inducible)
• Calculated Molecular Weight: 351 aa, 41 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC108261
• Gene Symbol: RRM2B
• Gene ID (NCBI): 50484
• RRID: AB_2878480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7LG56
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Ther; DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.; Authors - Fangfang Zhang ; KD Validated