RRP7A Polyclonal antibody

RRP7A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24490-1-AP

Synonyms

BK126B4.3, CGI 96, Gastric cancer antigen Zg14, RRP7A

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Validation Data Gallery

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  WB analysis of mouse thymus using 24490-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24490-1-AP (RRP7A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 24490-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 24490-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 24490-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 24490-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24490-1-AP (RRP7A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A431 using 24490-1-AP

  Immunofluorescent analysis of A431 cells using 24490-1-AP (RRP7A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse thymus tissue
• Positive IHC detected in: human brain tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24490-1-AP is RRP7A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RRP7A fusion protein Ag21636
• Full Name: ribosomal RNA processing 7 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 280 aa, 32 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC121118
• Gene Symbol: RRP7A
• Gene ID (NCBI): 27341
• RRID: AB_2879570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RRP7A, also named as Ribosomal RNA-processing protein 7 homolog A, is a 280 amino acid protein, which belongs to the RRP7 family. The calcualted molecular weight of RRP7A is 32 kDa, but modified RRP7A is about 42 kDa.