RSAD2 Polyclonal antibody

RSAD2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28089-1-AP

Synonyms

2510004L01Rik, cig33, cig5, RSAD2, vig1, Viperin



Tested Applications

Positive WB detected infetal human brain tissue, A549 cells, mouse spinal cord tissue
Positive IHC detected inmouse stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

28089-1-AP targets RSAD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSAD2 fusion protein Ag27733
Full Name radical S-adenosyl methionine domain containing 2
Calculated molecular weight 361 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC017969
Gene symbol RSAD2
Gene ID (NCBI) 91543
RRIDAB_2881057
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon.

Protocols

Product Specific Protocols
WB protocol for RSAD2 antibody 28089-1-APDownload protocol
IHC protocol for RSAD2 antibody 28089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production

Authors - Sayuri Sakuragi
humanIHC

Am J Cancer Res

Identification of potential immune-related circRNA-miRNA-mRNA regulatory network in cutaneous squamous cell carcinoma

Authors - Yuanfeng Wei
humanWB

Cell Mol Biol Lett

5-Methoxyflavone-induced AMPKα activation inhibits NF-κB and P38 MAPK signaling to attenuate influenza A virus-mediated inflammation and lung injury in vitro and in vivo

Authors - Sushan Yang
mouseWB

Front Nutr

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics.

Authors - Qiongxin Liang
mouseWB

J Appl Toxicol

Intratracheal instillation of graphene oxide decreases anti-virus responses and lipid contents via suppressing Toll-like receptor 3 in mouse livers.

Authors - Xiaomin Tang
humanWB,IF,IP

Biochemistry

Interactions between Viperin, Vesicle-Associated Membrane Protein A, and Hepatitis C Virus Protein NS5A Modulate Viperin Activity and NS5A Degradation.

Authors - Soumi Ghosh