RSPH3 Polyclonal antibody

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• RSPH3 Polyclonal antibody (17603-1-AP)

RSPH3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17603-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

RSP3, RSHL2, Radial spoke head-like protein 2, A-kinase anchor protein RSPH3

• 

  WB analysis of human brain using 17603-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17603-1-AP (RSPH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 17603-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17603-1-AP (RSPH3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17603-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17603-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 17603-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 17603-1-AP (RSPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue, NIH/3T3 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17603-1-AP targets RSPH3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11707

  Product name: Recombinant human RSPH3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 216-560 aa of BC050604

  Sequence: SLELQRQREARKRALARKQAQEQLRPQTPEPVEGRKHVDVQTELYLEEIADRIIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILEGELFDFDLEVKPVLEVLVGKTIEQSLLEVMEEEELANLRASQREYEELRNSERAEVQRLEEQERRHREEKERRKKQQWEIMHKHNETSQKIAARAFAQRYLADLLPSVFGSLRDSGYFYDPIERDIEIGFLPWLMNEVEKTMEYSMVGRTVLDMLIREVVEKRLCMYEHGEDTHQSPEPEDEPGGPGAMTESLEASEFLEQSMSQTRELLLDGGYLQRTTYDRRSSQERKFMEERELLGQDEETAMRKSLGEEELS

• Full Name: radial spoke 3 homolog (Chlamydomonas)
• Calculated Molecular Weight: 560 aa, 64 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC050604
• Gene Symbol: RSPH3
• Gene ID (NCBI): 83861
• RRID: AB_2181073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86UC2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RSPH3 Polyclonal antibody (Cat# 17603-1-AP) has 11 citations published in journals including Nature Communications, EBioMedicine, Science China Life Sciences, Cell Reports, Current Biology, Journal of Molecular Cell Biology, Frontiers in Endocrinology, Scientific Reports, Andrology, Journal of Assisted Reproduction and Genetics, and eLife. Research fields span cilia/flagella biology, radial spoke assembly, axonemal structure, and male infertility.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	CoIP	Nat Commun; Multi-scale structures of the mammalian radial spoke and divergence of axonemal complexes in ependymal cilia; Authors - Xueming Meng
mouse	WB	EBioMedicine; CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men; Authors - Hui-Juan Jin
mouse	WB,IF	Sci China Life Sci; Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes; Authors - Huan Wu
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
		J Mol Cell Biol; Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is essential for ciliary beat.; Authors - Yirong Zhang