RSPH4A Polyclonal antibody

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  WB analysis using 21931-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21931-1-AP (RSPH4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

RSPH4A Polyclonal Antibody for WB, ELISA

Cat No. 21931-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RSHL3, CILD11

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Tested Applications

• Positive WB detected in: mouse trachea tissue, rat trachea tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21931-1-AP targets RSPH4A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSPH4A fusion protein Ag16551
• Full Name: radial spoke head 4 homolog A (Chlamydomonas)
• Calculated Molecular Weight: 716 aa, 81 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC106949
• Gene Symbol: RSPH4A
• Gene ID (NCBI): 345895
• RRID: AB_3669381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5TD94
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSPH4A, also known as RSHL3, belongs to the radial spoke family. RSPH4A is the component of the axonemal radial spoke head which plays an important role in ciliary motility. Mutations in RSPH4A cause primary ciliary dyskinesia 1, a disease arising from dysmotility of motile cilia and sperm(PMID: 19200523, 23798057).