RSPH9 Polyclonal antibody

RSPH9 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 23253-1-AP

Synonyms

C6orf206, CILD12, MRPS18AL1, RSPH9

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Validation Data Gallery

• 

  WB analysis of mouse testis using 23253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 23253-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 23253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23253-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, NIH/3T3 cells, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 5 publications below

Product Information

The immunogen of 23253-1-AP is RSPH9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RSPH9 fusion protein Ag19745
• Full Name: radial spoke head 9 homolog (Chlamydomonas)
• Calculated Molecular Weight: 276 aa, 31 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC029519
• Gene Symbol: RSPH9
• Gene ID (NCBI): 221421
• RRID: AB_2879240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IF	Hum Mol Genet; Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse.; Authors - Jintao Zhang
		Eur J Hum Genet; Bi-allelic variants in human TCTE1/DRC5 cause asthenospermia and male infertility.; Authors - Shushu Zhou
mouse	WB,IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB	EBioMedicine; CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men; Authors - Hui-Juan Jin
human	IF	Cell; Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility; Authors - Lunni Zhou
mouse	WB	Elife; LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility; Authors - Jae Yeon Hwang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Célia (Verified Customer) (10-28-2024)

I tested 1/100 to be sure that it works in IF but the concentration was a little high so 1/200 should still be fine.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Spermatozoa