Tested Applications
| Positive WB detected in | mouse small intestine tissue, Transfected HEK-293 cells |
| Positive IP detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17193-1-AP targets RSPO3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9899 Product name: Recombinant human RSPO3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-272 aa of BC022367 Sequence: GRRQRRMHPNVSQGCQGGCATCSDYNGCLSCKPRLFFALERIGMKQIGVCLSSCPSGYYGTRYPDINKCTKCKADCDTCFNKNFCTKCKSGFYLHLGKCLDNCPEGLEANNHTMECVSIVHCEVSEWNPWSPCTKKGKTCGFKRGTETRVREIIQHPSAKGNLCPPTNETRKCTVQRKKCQKGERGKKGRERKRKKPNKGESKEAIPDSKSLESSKEIPEQRENKQQQKKRKVQDKQKSVSVSTVH Predict reactive species |
| Full Name | R-spondin 3 homolog (Xenopus laevis) |
| Calculated Molecular Weight | 272 aa, 31 kDa |
| Observed Molecular Weight | 30-40 kDa |
| GenBank Accession Number | BC022367 |
| Gene Symbol | RSPO3 |
| Gene ID (NCBI) | 84870 |
| RRID | AB_2878360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for RSPO3 antibody 17193-1-AP | Download protocol |
| WB protocol for RSPO3 antibody 17193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RSPO3 antibody (Cat# 17193-1-AP) has 19 citations published in journals including Nature, Cancer Discovery, Nature Communications, PNAS, Cardiovascular Research, and Cell Death & Disease. Research fields span Wnt signaling, tumor microenvironment and immunotherapy, liver zonation, cardiovascular disease, neuroprotection, diabetes-related vascular injury, wound healing, osteogenesis, gastric cancer, skeletal muscle regeneration, preeclampsia, and inflammatory arthritis.
| Species | Application | Title |
|---|---|---|
Cancer Discov Tumor Microenvironment-Derived R-spondins Enhance Antitumor Immunity to Suppress Tumor Growth and Sensitize for Immune Checkpoint Blockade Therapy | ||
Nat Commun R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine. | ||
J Exp Clin Cancer Res Subtypes in pancreatic ductal adenocarcinoma based on niche factor dependency show distinct drug treatment responses. | ||
Cell Death Dis Endothelial cell-derived RSPO3 activates Gαi1/3-Erk signaling and protects neurons from ischemia/reperfusion injury
|
Reviews
What customers say
One reviewer reported excellent performance in Western Blot at a 1:250 dilution, noting a clear band at the expected molecular weight that confirmed successful transfection. The product received a 5-star rating.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gerald (Verified Customer) (07-23-2025) | Worked very well for Western Blot, showing a clear band at the expected molecular weight size, thereby confirming a successful transfection.
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