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  • KD/KO Validated

RSPO3 Polyclonal antibody

RSPO3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17193-1-AP


CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, PWTSR, R spondin 3, Roof plate specific spondin 3, RSPO3, THSD2

Tested Applications

Positive WB detected inmouse small intestine tissue, Transfected HEK-293 cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17193-1-AP targets RSPO3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSPO3 fusion protein Ag9899
Full Name R-spondin 3 homolog (Xenopus laevis)
Calculated Molecular Weight 272 aa, 31 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC022367
Gene Symbol RSPO3
Gene ID (NCBI) 84870
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.


Product Specific Protocols
WB protocol for RSPO3 antibody 17193-1-APDownload protocol
IHC protocol for RSPO3 antibody 17193-1-APDownload protocol
IP protocol for RSPO3 antibody 17193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine.

Authors - Teng Han

Proc Natl Acad Sci U S A

RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.

Authors - Kendra S Carmon

Cell Death Dis

Endothelial cell-derived RSPO3 activates Gαi1/3-Erk signaling and protects neurons from ischemia/reperfusion injury

Authors - Ting-Tao Liu
  • KD Validated

Cardiovasc Res

Role of R-spondin 2 in arterial lymphangiogenesis and atherosclerosis.

Authors - Bhupesh Singla

Am J Physiol Cell Physiol

R-Spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells.

Authors - Zhilong Chen

J Exp Clin Cancer Res

Subtypes in pancreatic ductal adenocarcinoma based on niche factor dependency show distinct drug treatment responses.

Authors - Tomohiko Shinkawa