RSPO4 Polyclonal antibody

RSPO4 Polyclonal Antibody for WB, ELISA

Cat No. 28559-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

C20orf182, CRISTIN4, dJ824F16.3, FLJ16018, hRspo4, R spondin 4, R spondin family, member 4, Roof plate specific spondin 4, RSPO4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis using 28559-1-AP
Rat brain tissue lysates were subjected to SDS PAGE followed by western blot with 28559-1-AP (RSPO4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

rat brain tissue

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

28559-1-AP targets RSPO4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag29308 Product name: Recombinant human RSPO4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 163-234 aa of NM_001029871 Sequence: LESRVREAGRAGHEEAATCQVLSESRKCPIQRPCPGERSPGQKKGRKDRRPRKDRKLDRRLDVRPRQPGLQP Predict reactive species
Full Name	R-spondin family, member 4
Calculated Molecular Weight	26 kDa
Observed Molecular Weight	~19 kDa
GenBank Accession Number	NM_001029871
Gene Symbol	RSPO4
Gene ID (NCBI)	343637
RRID	AB_2881170
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q2I0M5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for RSPO4 antibody 28559-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
		Pharmacol Res Targeted MSC therapy restores implantation by spatially reconstituting the RSPO1 niche via IL10-CXCL12 signaling. Authors - Yue Dang View Article