RTCD1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• RTCD1 Polyclonal antibody (15996-1-AP)

• Featured Product
• KD/KO Validated

RTCD1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15996-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RNA 3 phosphate cyclase, RNA cyclase, RPC, RPC1, RTC1, RTCD1

• 

  WB analysis using 15996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15996-1-AP (RTCD1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 15996-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15996-1-AP (RTCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 15996-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15996-1-AP (RTCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 15996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15996-1-AP (RTCD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 15996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15996-1-AP (RTCD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for RTCD1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HL-60 cells, Jurkat cells, K-562 cells, THP-1 cells, U-251 cells, U-87 MG cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15996-1-AP targets RTCD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8904

  Product name: Recombinant human RTCD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-379 aa of BC012604

  Sequence: MAGPRVEVDGSIMEGGGQILRVSTALSCLLGLPSRVQKIRAGRSTPGLSSGGWKSKIKVLTRPQHLSGLEMIRDLCDGQLEGAEIGSTEITFTPEKIKGGIHTADTKTAGSVCLLMQVSMPCVLFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGYYPKGGGEVIVRMSPVKQLNPINLTERGCVTKIYGRAFVAGVLPFKVAKDMAAAAVRCIRKEIRDLYVNIQPVQEPKDQAFGNGNGIIIIAETSTGCLFAGSSLGKRGVNADKVGIEAAEMLLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQIAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL

• Full Name: RNA terminal phosphate cyclase domain 1
• Calculated Molecular Weight: 379 aa, 41 kDa
• Observed Molecular Weight: 41-43 kDa
• GenBank Accession Number: BC012604
• Gene Symbol: RTCD1
• Gene ID (NCBI): 8634
• RRID: AB_2180923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00442
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RTCD1 polyclonal antibody (Cat# 15996-1-AP) has 1 total citation. It was cited in a study published in Journal of Biological Chemistry (IF 4.1, 2018), which investigated how the cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress. The research field encompasses endoplasmic reticulum stress responses, RNA splicing regulation, and phosphodiesterase biology in human cells. The antibody was applied in Western blot with RNAi validation.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Biol Chem; The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.; Authors - Irem Unlu ; KD Validated