Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31232-1-AP targets RTTN in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34960 Product name: Recombinant human RTTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1417-1530 aa of NM_173630 Sequence: TVVNILLDQSECSMVRREAAFILQNLLVIPMPTEIIKDYTWQGPCVHDEDSGLSLIGKPALQALLYHCHFYEHLNQMVKHCYLGRCMFDLNFSAFDRNSESNDLNGLDDSFKFW Predict reactive species |
| Full Name | rotatin |
| Calculated Molecular Weight | 249 kDa |
| Observed Molecular Weight | 246 kDa |
| GenBank Accession Number | NM_173630 |
| Gene Symbol | RTTN |
| Gene ID (NCBI) | 25914 |
| RRID | AB_3742412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86VV8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RTTN, also known as Rotatin, is an evolutionarily conserved protein that is associated with the centrosome and plays a crucial role in centriole biogenesis. It is found in many organisms and was initially identified in a mutant mouse that exhibited defects in axial rotation and left-right specification. RTTN is required for proper mitotic progression, the maintenance of spindle pole integrity, and correct spindle positioning. Loss of RTTN can lead to mitotic defects, including G2/M phase arrest and increased aneuploidy cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RTTN antibody 31232-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
