RUFY1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85839-4-RR (same clone as 85839-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85839-4-RR (RUFY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85839-4-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 85839-4-RR (same clone as 85839-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RUFY1 antibody (85839-4-RR, Clone: 250159A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85839-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85839-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85839-4-RR against Human RUFY1 were performed. The affinity constant is 0.444 nM.

RUFY1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85839-4-PBS

Clone No.250159A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, RABIP4, RUFY 1

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Product Information

85839-4-PBS targets RUFY1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4324

  Product name: Recombinant human RUFY1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 219-600 aa of BC032571

  Sequence: TKIDGLEKTNSKLQEELSAATDRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGKTNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQALQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEVNQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS

• Full Name: RUN and FYVE domain containing 1
• Calculated Molecular Weight: 708 aa, 80 kDa
• Observed Molecular Weight: 80 kDa, 69 kDa
• GenBank Accession Number: BC032571
• Gene Symbol: RUFY1
• Gene ID (NCBI): 80230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96T51
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.