RUFY2 Polyclonal antibody

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  WB analysis of PC-3 using 14137-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14137-1-AP (RUFY2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14137-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14137-1-AP (RUFY2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

RUFY2 Polyclonal Antibody for WB, ELISA

Cat No. 14137-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA1537, RABIP4R, RUFY2, ZFYVE13

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14137-1-AP targets RUFY2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5363

  Product name: Recombinant human RUFY2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-403 aa of BC041092

  Sequence: MATKDPTAVERANLLNMAKLSIKGLIESALSFGRTLDSDYPPLQQFFVVMEHCLKHGLKVRKSFLSYNKTIWGPLELVEKLYPEAEEIGASVRDLPGLNEFYEYHALMMEEEGAVIVGLLVGLNVIDANLCVKGEDLDSQVGVIDFSMYLKNEEDIGNKERNVQIAAILDQKNYVEELNRQLNSTVSSLHSRVDSLEKSNTKLIEELAIAKNNIIKLQEENHQLRSENKLILMKTQQHLEVTKVDVETELQTYKHSRQGLDEMYNEARRQLRDESQLRQDVENELAVQVSMKHEIELAMKLLEKDIHEKQDTLIGLRQQLEEVKAINIEMYQKLQGSEDGLKEKNEIIARLEEKTNKITAAMRQLEQSDNDLLTQTRTIAMSLVKCASSDTQDQYKLVKDISF

• Full Name: RUN and FYVE domain containing 2
• Calculated Molecular Weight: 403 aa, 46 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC041092
• Gene Symbol: RUFY2
• Gene ID (NCBI): 55680
• RRID: AB_10643375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WXA3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.