RUFY2 Polyclonal antibody

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• RUFY2 Polyclonal antibody (19180-1-AP)

RUFY2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19180-1-AP Copied!

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ZFYVE13, RUN and FYVE domain-containing protein 2, RABIP4R, Rab4-interacting protein related, KIAA1537

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  WB analysis of mouse lung using 19180-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 19180-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 19180-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 19180-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 19180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 19180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19180-1-AP (RUFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 19180-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19180-1-AP (RUFY2 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse lung tissue, mouse ovary tissue, SH-SY5Y cells, U-251 cells, rat lung tissue
• Positive IHC detected in: human testis tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19180-1-AP targets RUFY2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5938

  Product name: Recombinant human RUFY2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-403 aa of BC041092

  Sequence: MATKDPTAVERANLLNMAKLSIKGLIESALSFGRTLDSDYPPLQQFFVVMEHCLKHGLKVRKSFLSYNKTIWGPLELVEKLYPEAEEIGASVRDLPGLNEFYEYHALMMEEEGAVIVGLLVGLNVIDANLCVKGEDLDSQVGVIDFSMYLKNEEDIGNKERNVQIAAILDQKNYVEELNRQLNSTVSSLHSRVDSLEKSNTKLIEELAIAKNNIIKLQEENHQLRSENKLILMKTQQHLEVTKVDVETELQTYKHSRQGLDEMYNEARRQLRDESQLRQDVENELAVQVSMKHEIELAMKLLEKDIHEKQDTLIGLRQQLEEVKAINIEMYQKLQGSEDGLKEKNEIIARLEEKTNKITAAMRQLEQSDNDLLTQTRTIAMSLVKCASSDTQDQYKLVKDISF

• Full Name: RUN and FYVE domain containing 2
• Calculated Molecular Weight: 403 aa, 46 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC041092
• Gene Symbol: RUFY2
• Gene ID (NCBI): 55680
• RRID: AB_10643382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WXA3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product is highly positive, rated 5 stars. The reviewer used the antibody at a 1:1000 dilution for Western Blot and reported that it "works well." Overall, the feedback indicates reliable performance for Western Blot applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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sarah (Verified Customer) (01-02-2025)

works well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000