RUFY3 Polyclonal antibody

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• RUFY3 Polyclonal antibody (15383-1-AP)

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  WB analysis using 15383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15383-1-AP (RUFY3 antibody) at dilution of 1:300000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 15383-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15383-1-AP (RUFY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15383-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15383-1-AP (RUFY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 15383-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RUFY3 antibody (15383-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

RUFY3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15383-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0871, Protein RUFY3, Rap2 interacting protein x, Rap2-interacting protein x, RIPx

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15383-1-AP targets RUFY3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5191

  Product name: Recombinant human RUFY3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 110-469 aa of BC051716

  Sequence: HCLKHGLKAKKTFLGQNKSFWGPLELVEKLVPEAAEITASVKDLPGLKTPVGRGRAWLRLALMQKKLSEYMKALINKKELLSEFYEPNALMMEEEGAIIAGLLVGLNVIDANFCMKGEDLDSQVGVIDFSMYLKDGNSSKGTEGDGQITAILDQKNYVEELNRHLNATVNNLQAKVDALEKSNTKLTEELAVANNRIITLQEEMERVKEESSYILESNRKGPKQDRTAEGQALSEARKHLKEETQLRLDVEKELEMQISMRQEMELAMKMLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSRLEEKTNQMAATIKQLEQSEKDLVKQAKTLNSAANKLIPKHH

• Full Name: RUN and FYVE domain containing 3
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa, 65 kDa
• GenBank Accession Number: BC051716
• Gene Symbol: RUFY3
• Gene ID (NCBI): 22902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L099
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUFY3 (RUN and FYVE domain-containing protein 3) was originally described as lacking part of the C-terminal CC2 and FYVE domains. The interaction of RUFY3 with the filamentous actin network through a complex formed together with Rap2 and Fascin 1 (FSCN1) is critical for axonogenesis and growth cone development (PMID: 37463962). RUFY3 also seems to play a crucial role in promoting cancer development (PMID: 34510031).