RUNDC3B Polyclonal antibody

RUNDC3B Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17113-1-AP

Synonyms

Rap2 binding protein 9, Rap2 interacting protein 9, RPIB9, RPIP 9, RPIP9, RUN domain containing 3B, RUNDC3B

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Validation Data Gallery

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  WB analysis of MCF-7 using 17113-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17113-1-AP (RUNDC3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17113-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17113-1-AP (RUNDC3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 17113-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17113-1-AP (RUNDC3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, mouse brain tissue
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17113-1-AP is RUNDC3B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RUNDC3B fusion protein Ag10797
• Full Name: RUN domain containing 3B
• Calculated Molecular Weight: 387 aa, 43 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC022520
• Gene Symbol: RUNDC3B
• Gene ID (NCBI): 154661
• RRID: AB_10699872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.