RUNX1 (N-terminal) Polyclonal antibody

RUNX1 (N-terminal) Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19555-1-AP

Synonyms

AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1, RUNX1 (N-terminal)

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Validation Data Gallery

• 

  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19555-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 19555-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19555-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19555-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19555-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19555-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19555-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19555-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19555-1-AP

  IP result of anti-RUNX1 (N-terminal) (IP:19555-1-AP, 4ug; Detection:19555-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue, HEK-293 cells, HL-60 cells, Jurkat cells, MCF-7 cells, mouse spleen tissue, mouse thymus tissue, Raji cells, SH-SY5Y cells, Sp2/0 cells
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

19555-1-AP targets RUNX1 (N-terminal) in WB, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: runt-related transcription factor 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: NM_001754
• Gene Symbol: RUNX1
• Gene ID (NCBI): 861
• RRID: AB_10949190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa. This antibody (19555-1-AP) can't recognize 37 kDa isoform 11.

Publications

Species	Application	Title
mouse	WB	Protein Expr Purif; Msx1 cooperates with Runx1 for inhibiting myoblast differentiation.; Authors - Guoqiang Zhou
human	WB,CoIP	Mol Cells; RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15; Authors - Hao Ding
rat	ChIP,WB	Respir Res; RUNX1 targeting AKT3 promotes alveolar hypercoagulation and fibrinolytic inhibition in LPS induced ARDS; Authors - Chuan Xiao