RUNX1 (N-terminal) Polyclonal antibody

RUNX1 (N-terminal) Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 19555-1-AP

Synonyms

AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, HL-60 cells, Jurkat cells, MCF-7 cells, mouse spleen tissue, mouse thymus tissue, Raji cells, SH-SY5Y cells, Sp2/0 cells
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

19555-1-AP targets RUNX1 (N-terminal) in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name runt-related transcription factor 1
Calculated molecular weight 51 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberNM_001754
Gene symbol RUNX1
Gene ID (NCBI) 861
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Runt-related transcription factor 1 (RUNX1), also named as AML1 or CBF alpha 2, is 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists some isoforms with range of MV 20-52 kDa. The calculated molecular weight of isoforms 1 is 49 kDa, but the modified protein is about 49-55 kDa. This antibody (19555-1-AP) can't recognize 37 kDa isoform 11.

Protocols

Product Specific Protocols
WB protocol for RUNX1 (N-terminal) antibody 19555-1-APDownload protocol
IP protocol for RUNX1 (N-terminal) antibody 19555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Protein Expr Purif

Msx1 cooperates with Runx1 for inhibiting myoblast differentiation.

Authors - Guoqiang Zhou