RUNX1T1 Monoclonal antibody

RUNX1T1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G2F7

Cat no : 67086-1-Ig

Synonyms

AML1T1, CBFA2T1, CDR, Cyclin D related protein, Eight twenty one protein, ETO, MTG8, MTG8b, Protein CBFA2T1, Protein ETO, Protein MTG8, RUNX1T1, ZMYND2

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Validation Data Gallery

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  WB analysis of HEK-293 using 67086-1-Ig

  WB result of RUNX1T1 antibody (67086-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RUNX1T1 transfected HEK-293 cells.

• 

  WB analysis of Jurkat using 67086-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67086-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67086-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67086-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67086-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67086-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67086-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67086-1-Ig (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, pig brain tissue, K-562 cells, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67086-1-Ig targets RUNX1T1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RUNX1T1 fusion protein Ag7893
• Full Name: runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC005850
• Gene Symbol: RUNX1T1
• Gene ID (NCBI): 862
• RRID: AB_2882394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. Various transcript of the fusion gene has been reported. RUNX1T1 exists some isoforms with MV 68, 67,64, 48 and 44 kDa. The calcualted molecular weight of RUNX1T1 is 67 kDa, but modified RUNX1T1is about 70-75 kDa.