RUNX2 Polyclonal antibody

RUNX2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, chIP

Conjugate

Unconjugated

Cat no : 20700-1-AP

Synonyms

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2



Tested Applications

Positive WB detected inROS1728 cells
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20700-1-AP targets RUNX2 in WB, IHC, IF, chIP applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name runt-related transcription factor 2
Calculated molecular weight 57 kDa
Observed molecular weight 57-60 kDa
GenBank accession numberNM_004348
Gene symbol RUNX2
Gene ID (NCBI) 860
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

Protocols

Product Specific Protocols
WB protocol for RUNX2 antibody 20700-1-APDownload protocol
IF protocol for RUNX2 antibody 20700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,WB

Arterioscler Thromb Vasc Biol

Potential Role of H-Ferritin in Mitigating Valvular Mineralization.

Authors - Katalin Éva Sikura
mouseWB

J Mech Behav Biomed Mater

Citric acid enhances the physical properties, cytocompatibility and osteogenesis of magnesium calcium phosphate cement.

Authors - Shuang Wang
ratWB

J Cell Physiol

Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.

Authors - Yan-Jing Zhang
mouseWB

BMC Oral Health

ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum.

Authors - Jingyu Wang
humanWB

Ann Transl Med

Inhibition of pyroptosis attenuates Staphylococcus aureus-induced bone injury in traumatic osteomyelitis.

Authors - Xiaobo Zhu
ratWB

Int J Nanomedicine

The Role of Tantalum Nanoparticles in Bone Regeneration Involves the BMP2/Smad4/Runx2 Signaling Pathway.

Authors - Guilan Zhang