RUNX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RUNX2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

85

Cat no : 20700-1-AP

Synonyms

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

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Validation Data Gallery

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  WB analysis of ROS1728 using 20700-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20700-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 20700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20700-1-AP (RUNX2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: ROS1728 cells, MCF-7 cells
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 74 publications below
• IHC: See 9 publications below
• IF: See 13 publications below
• ChIP: See 1 publications below

Product Information

20700-1-AP targets RUNX2 in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: runt-related transcription factor 2
• Calculated molecular weight: 57 kDa
• Observed molecular weight: 57-60 kDa
• GenBank accession number: NM_004348
• Gene symbol: RUNX2
• Gene ID (NCBI): 860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

Publications

Species	Application	Title
	WB	ACS Appl Mater Interfaces; Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis; Authors - Congcong Yu
mouse	IHC	Clin Immunol; STING contributes to trauma-induced heterotopic ossification through NLRP3-dependent macrophage pyroptosis; Authors - Ziyang Sun
mouse	WB,IF	Br J Pharmacol; Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease.; Authors - Katalin Éva Sikura
human	WB	BMC Med; Loss of thymidine phosphorylase activity disrupts adipocyte differentiation and induces insulin-resistant lipoatrophic diabetes.; Authors - Jérémie Gautheron
human	WB	Arterioscler Thromb Vasc Biol; Hypoxia Triggers Osteochondrogenic Differentiation of Vascular Smooth Muscle Cells in an HIF-1 (Hypoxia-Inducible Factor 1)-Dependent and Reactive Oxygen Species-Dependent Manner.; Authors - Enikő Balogh
mouse	WB	Int J Mol Sci; Atf7ip Inhibits Osteoblast Differentiation via Negative Regulation of the Sp7 Transcription Factor; Authors - Guoqin Hu

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