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- KD/KO Validated
RUNX2 Polyclonal antibody
RUNX2 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
WB, IHC, IF, chIP, ELISA
Conjugate
Unconjugated
Cat no : 20700-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | ROS1728 cells, MCF-7 cells |
Positive IF detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 74 publications below |
IHC | See 9 publications below |
IF | See 13 publications below |
ChIP | See 1 publications below |
Product Information
20700-1-AP targets RUNX2 in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat, chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | runt-related transcription factor 2 |
Calculated molecular weight | 57 kDa |
Observed molecular weight | 57-60 kDa |
GenBank accession number | NM_004348 |
Gene symbol | RUNX2 |
Gene ID (NCBI) | 860 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.
Protocols
Product Specific Protocols | |
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WB protocol for RUNX2 antibody 20700-1-AP | Download protocol |
IF protocol for RUNX2 antibody 20700-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Appl Mater Interfaces Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis | ||
Clin Immunol STING contributes to trauma-induced heterotopic ossification through NLRP3-dependent macrophage pyroptosis | ||
Br J Pharmacol Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease. | ||
BMC Med Loss of thymidine phosphorylase activity disrupts adipocyte differentiation and induces insulin-resistant lipoatrophic diabetes. | ||
Arterioscler Thromb Vasc Biol Hypoxia Triggers Osteochondrogenic Differentiation of Vascular Smooth Muscle Cells in an HIF-1 (Hypoxia-Inducible Factor 1)-Dependent and Reactive Oxygen Species-Dependent Manner. | ||
Int J Mol Sci Atf7ip Inhibits Osteoblast Differentiation via Negative Regulation of the Sp7 Transcription Factor |